St. Jude has a long history of innovation in the treatment of pediatric cancer. Less well known is the work performed at St. Jude to study the long-term impact of the treatment regimens on survivors of pediatric cancer. Many of these effects such as cognitive or physical impairments are immediately obvious. Recently, the examination of molecular markers as a predictor or indicator of future health issues in pediatric cancer survivors has begun to be investigated. For example, certain treatment protocols associated with Hodgkin's Lymphoma are associated with a change in the makeup of cell populations within the peripheral blood that includes genetic alterations correlated with an increased risk of breast cancer. There is also increasing evidence that the probability of the early onset of frailty can be predicted by measuring specific sites of methylation on certain populations of blood cells by what is popularly referred to as an epigenetic aging clock. The goal of our work will be to build on these early studies, by increasing the number of patients to better understand these biological mechanisms among large survivor populations and expand the scope of genes and modified sites examined to identify additional predictors of current and future adverse health outcomes. These projects will also evaluate samples from patients at both the population and single-cell levels to determine if specific cell populations or single-cell programs serve as more accurate predictors of health outcomes.

Many of the projects involve large sample sets, requiring extremely meticulous and accurate handling of samples.

Responsibilities

• Perform accurate and precise sample processing, including RNA Isolation, DNA isolation, preparation of single-cell suspensions, and archiving of material under the supervision of the operations manager.
• Prepare libraries and perform sequencing on NGS platforms. Data types include but are not limited to WGS, RNASeq, WES, and single-cell multi-omics.

Opportunities for Professional Growth

• The selected candidate will have the opportunity to learn the protocols for NGS library preparation and post-analysis processes used for Next Generation Sequencing.

Preferred Skills:

• Experience in basic molecular biology such as PCR, QPCR, DNA isolation, and RNA isolation.
• Familiarity with Microsoft Excel, PowerPoint, and Word software.

Minimum Education and/or Training:

• Bachelor's degree in a relevant scientific area is required.

Minimum Experience:

• Two (2) years of relevant, post-degree work experience is required.
• No experience is required with a Master's degree.

Compensation

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Diversity, Equity and Inclusion

St. Jude Children's Research Hospital has a diverse, global patient population and workforce, built on the principles of diversity, equity and inclusion. Our founder Danny Thomas envisioned a hospital that would treat children of the world-regardless of race, religion or a family's ability to pay. .

Today, we continue the mission to advance cures and means of prevention for pediatric catastrophic diseases through research and treatment. As we accelerate this progress globally, we believe our legacy of diversity, equity and inclusion is foundational to success. With the commitment of leaders at all levels of the organization, we strive to ensure the St. Jude culture, leadership approaches and talent processes are equitable and culturally responsive. View our to learn about the hospital's roots in diversity, equity and inclusion, where we are today and our aspirations for an even better future.

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St. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.